研究领域肿瘤  细胞生物  信号转导  干细胞  细胞周期蛋白  转录调节因子  
抗体来源Rabbit
克隆类型Monoclonal
克 隆 号6C5
交叉反应Human, Mouse,  (predicted: Rat, )
产品应用WB=1:500-2000 IHC-P=1:50-200 IHC-F=1:50-200 Flow-Cyt=1ug/Test ICC=1:100 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量56kDa
细胞定位细胞核 
性    状Liquid
浓    度1mg/ml
免 疫 原Recombinant protein within human KLF4 aa 1-500 
亚    型IgG
纯化方法affinity purified by Protein A
缓 冲 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMedPubMed
产品介绍KLF4 is a transcription factor that works with Sp1 to activate the Laminin gamma1 chain gene. It binds the CACCC core sequence. KLF4 may be involved in the differentiation of epithelial cells and may also function in the development of the skeleton and kidney. KLF4 also has roles as both an oncogene and a tumor suppressor.

Function:
Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.

Subunit:
Interacts with POU5F1/OCT4 and SOX2. Interacts with MUC1 (via the C-terminal domain).

Subcellular Location:
Nucleus.

Post-translational modifications:
Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.

Similarity:
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.

SWISS:
O43474

Gene ID:
9314