研究领域肿瘤  神经生物学  信号转导  通道蛋白  
抗体来源Rabbit
克隆类型Monoclonal
克 隆 号13H5
交叉反应Human, Mouse, Rat, 
产品应用WB=1:500-2000 IHC-P=1:50-100 IHC-F=1:50-100 Flow-Cyt=1:50 ICC=1:50 IF=1:50-100 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量113kDa
细胞定位细胞膜 
性    状Liquid
浓    度1mg/ml
免 疫 原Recombinant human Sodium Potassium ATPase aa 1-100 
亚    型IgG
纯化方法affinity purified by Protein A
缓 冲 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMedPubMed
产品介绍The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+-ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May2009].

Function:
This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.

Subunit:
Interacts with SIK1. Composed of three subunits: alpha (catalytic), beta and gamma. Binds the HLA class II histocompatibility antigen, DR1.

Subcellular Location:
Cell membrane; Multi-pass membrane protein. Melanosome.

Tissue Specificity:
Found in most tissues.

Post-translational modifications:
Phosphorylation on Tyr-10 modulates pumping activity. Dephosphorylation by protein phosphatase 2A (PP2A) following increases in intracellular sodium, leading to increase catalytic activity.

Similarity:
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.

SWISS:
P05023

Gene ID:
476